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Experimental Microscopy

 


Research Overview
experimental-microscopy1aThe newly established Experimental Microscopy Sub-platform (EMSP) provides collaborative and advisory assistance for scientists in Singapore Eye Research Institute/Singapore National Eye Center to use high resolution imaging for their research programs. To conduct fundamental and advanced research relating studies of morphological and expression characteristics to biological and material properties, EMSP is equipped with “state of the art” light, stereo and confocal microscopes.

Facilities

  • Carl Zeiss AXIOVERT 200 Motorized Microscope (AxioVision 4.8)
  • Carl Zeiss AXIOPLAN 2 Imaging Microscope (AxioVision 4.8)
  • Carl Zeiss AXIO Imager System with ApoTome (AxioVision 4.7.1)
  • Nikon C2 Confocal Laser Point Scanning System (NISElementsAr-4)
  • BioRad ZOE Fluorescence Cell Imager
  • Nikon SMZ1500 Stereomicroscope
  • Nikon Eclipse TS100-F Stereomicroscopes (NIS-Elements D)

Most of them are outfitted with digital imaging to allow investigator to quickly use images for manuscripts, presentations and grants.

Exp Microscopy1

Capabilities

  • Live cell and time-lapse imaging (cellular dynamics for biological functions)
  • Fluorescence microscopy (target protein expression in sub-cellular structures and single molecule tracking and quantification)
  • Phase contrast / differential interference contrast DIC microscopy (contrast-enhanced topographic imaging)
  • Confocal microscopy (high-resolution point scanning imaging for 3D image reconstruction)
  • Polarized light microscopy (anisotropy, birefringence composition)
  • Stereomicroscopy (for close work and micro-dissection)

Manpower

This platform has a team supported by Scientists, Research Officers and Laboratory Executives, who are committed to ensuring a high standard of research excellence and facility support.

Exp Microscopy2

Collaborators

  • Advanced Bio-imaging Core Platform, Academia, SingHealth
  • Electron Microscopy Unit, National University of Singapore (transmission and scanning electron microscopy, atomic force microscopy, cryosectioning and immunoelectron microscopy)
  • NanoCore, National University of Singapore (helium ion microscopy)
  • Institute of Molecular and Cell Biology, A*STAR Singapore (laser capture micro-dissection)

Morphological visualization in accompany with the dynamic biological processes is the backbone to most biological research. The EMSP supports image-related research, offering advices and technology support on various microscopies and image analysis resources.

We participate in different research projects involving:

  • Ocular tissue imaging (eg. corneal stromal collagen fibrillation, corneal endothelial cells)

  • Ultrastructural morphology and morphometric analysis

  • Protein expression on cells and tissues; co-localization analysis & quantification; 3D imaging

  • Live cell imaging and time-lapse protein expression and trafficking

  • Tissue dissection

  1. Lee MC, et al. Expression of the primary angle closure glaucoma susceptibility gene PLEKHA7 in endothelial and epithelial cell junctions in the eye. Invest Ophthalmol Vis Sci. 2014 May 55(6):3833-41.

  2. Yam GH, et al. Ex vivo propagation of human corneal stromal “activated keratocytes” for tissue engineering. Cell Transplantation 24(9), 1845-61 (2015).

  3. Chaurasia SS, et al. Hevin plays a pivotal role in corneal wound healing. PLoS One. 2013 Nov 26;8(11):e81544.

  4. Zhang T, et al. The effect of amniotic membrane de-epithelialization method on its biological properties and ability to promote limbal epithelial cell culture. Invest Ophthalmol Vis Sci. 2013 Apr 30;54(4):3072-81.

  5. Tan XW, et al. In vivo biocompatibility of two PEG/PAA interpenetrating polymer networks as corneal inlays following deep stromal pocket implantation. J Mater Sci Mater Med. 2013 Apr;24(4):967-77.

  6. Chaurasia SS, et al. Effect of fibrin glue on biomechanical properties of human Descemet’s membrane. PLoS One. 2012;7(5):e37456.

  7. Seet LF, et al. Involvement of SPARC and MMP-3 in pathogenesis of human pterygium. Invest Ophthalmol Vis Sci. 2012 Feb 2;53(2):587-95.

  8. Peh GS, et al. Cultivation of human corneal endothelial cells isolated from paired donor corneas. PLoS One. 2011;6(12):e28310.

  9. Riau AK, et al. Nanoscale helium ion microscopic analysis of collagen fibrillar changes following femtosecond laser dissection of human cornea. J Biomed Nanotechnol. 2014 Aug;10(8):1552-62.


Head: Dr Gary Yam
Contact Us: gary.yam.h.f@seri.com.sg